TLR5 antibody (Middle Region)

Catalog No. ABIN265143

The Rabbit Polyclonal anti-TLR5 antibody (ABIN265143) specifically detects TLR5 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TLR5 antibody (Middle Region) (ABIN265143)

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TLR5 Antibody

Binding Specificity: Middle Region

Specificity: This antibody detects TLR5. Found in the membrane, TLR5 is hghly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis (6).

Purification: Purified

Immunogen: Peptide corresponding to 16 amino acids near the center of human TLR5

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 to 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for TLR5

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Alternative Name: TLR5

Background: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-? and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires' disease and demonstrates the importance of TLR5 in the innate immune response (5).Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Gene ID: 7100

NCBI Accession: NP_003259

UniProt: O60602

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades