EEF2 antibody

Catalog No. ABIN265392

The Rabbit Polyclonal anti-EEF2 antibody (ABIN265392) specifically detects EEF2 in WB, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EEF2 antibody (ABIN265392)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EEF2 Antibody

Specificity: This antibody detects endogenous levels of eEF2 protein. (region surrounding Ala50)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2

Background: Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved.Synonyms: EF-2, EF2, Elongation factor 2

Molecular Weight: approx. 100 kDa

Gene ID: 1938

NCBI Accession: NP_001952

UniProt: P13639

Pathways: AMPK Signaling