Ephrin B3 antibody

Catalog No. ABIN265403

The Rabbit Polyclonal anti-Ephrin B3 antibody is suitable to detect Ephrin B3 in samples from Mouse, Human and Rat. It has been validated for WB and IHC (p).

Quick Overview for Ephrin B3 antibody (ABIN265403)

Target: Ephrin B3 (EFNB3)

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ephrin B3 Antibody

Specificity: This antibody detects endogenous levels of Ephrin-B3 protein. (region surrounding Trp251)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Ephrin B3

Target: Ephrin B3 (EFNB3)

Alternative Name: Ephrin-B3

Background: Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors, Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors.Synonyms: EFNB3, ELK-L3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, EPLG8, LERK-8, LERK8

Molecular Weight: approx. 36 kDa

Gene ID: 1949

NCBI Accession: NP_001397

UniProt: Q15768

Pathways: RTK Signaling