HVEM antibody (PE-Cy7)

Catalog No. ABIN2659202

The Mouse Monoclonal anti-HVEM antibody has been validated for WB, FACS, IHC and IF. It is suitable to detect HVEM in samples from Human.

Quick Overview for HVEM antibody (PE-Cy7) (ABIN2659202)

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HVEM antibody is conjugated to PE-Cy7

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 122

Product Details anti-HVEM Antibody (PE-Cy7)

Characteristics: The 122 antibody recognizes human HVEM also known as herpesvirus entry mediator A, tumor necrosis factor receptor superfamily, member 14, TNFRSF14, and tumor necrosis factor receptor like 2. HVEM, a member of the TNFR superfamily, is a type I transmembrane protein containing 2 TNF receptor domains with a predicted molecular weight of approximately 30 kD. HVEM is widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen, thymus and other organs. Resting T cells and naive and memory B cells express high levels of HVEM as well. In humans, HVEM is not expressed in germinal center B cells. Immature dendritic cells express high levels of HVEM that is downregulated upon maturation. HVEM plays an important role in herpes simplex virus pathogenesis by enhancing entry into cells. Signaling through HVEM activates JNK1, NF-κB and AP-1 to control gene expression in response to infection or cellular stress and activate the immune response. HVEM binds to LIGHT and has also been shown to associate with several other proteins including TRAF1, TRAF2, TRAF3, TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha.

Purification: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for HVEM

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Alternative Name: CD270

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints