RUNX1 antibody

Catalog No. ABIN271868

The Rabbit Polyclonal anti-RUNX1 antibody is suitable to detect RUNX1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for RUNX1 antibody (ABIN271868)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RUNX1 Antibody

Specificity: This antibody detects endogenous levels of AML1 protein. (region surrounding His297)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 4000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background: AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors. It is required for normal development of all hematopoietic lineages. AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8,21) AML1-ETO, t(12,21) TEL-AML, and t(8,21) AML-M2. Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner.Synonyms: 1, AML-1, AML1, AML1-EVI-1, AMLCR1, Acute myeloid leukemia 1 protein, CBF-alpha-2, CBFA2, Core-binding factor subunit alpha-2, EVI-1, PEA2-alpha B, PEBP2-alpha B, PEBP2A2, Polyomavirus enhancer-binding protein 2 alpha B subunit, Runt-related transcription factor, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit

Molecular Weight: approx. 55 kDa

Gene ID: 861

NCBI Accession: NP_001001890

UniProt: Q01196