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RUNX1 antibody (AA 227-255)

The Rabbit Polyclonal anti-RUNX1 antibody is suitable to detect RUNX1 in samples from Human. It has been validated for WB, IF, FACS and IHC (p).

All lanes : Anti-RUNX1 Antibody at 1:2000 dilution Lane 1: S whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 μg per lane. (RUNX1 antibody (AA 227-255))

RUNX1 Antibody (ABIN650732 and ABIN2839428) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (RUNX1 antibody (AA 227-255))

FACS

Confocal immunofluorescent analysis of RUNX1 Antibody (ABIN650732 and ABIN2839428) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (RUNX1 antibody (AA 227-255))

RUNX1 Antibody (ABIN650732 and ABIN2839428) western blot analysis in U-937 cell line lysates (35 μg/lane). (RUNX1 antibody (AA 227-255))

Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (ABIN650732 and ABIN2839428) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RUNX1 antibody (AA 227-255))

IHC (p)

5 images

Catalog No. ABIN650732

$451.00

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Quick Overview for RUNX1 antibody (AA 227-255) (ABIN650732)

Target

See all RUNX1 Antibodies

RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This RUNX1 antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB15210

Discover our top product RUNX1 Primary Antibody

Binding Specificity
29

16

14

12

9

9

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 227-255

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This RUNX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from human RUNX1.

Isotype

Ig Fraction
anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 210-310) antibody
RUNX1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 2C10 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 150-250) antibody
RUNX1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 200-233), (Middle Region) antibody
RUNX1 Reactivity: Human, Mouse, Rat WB, IHC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 210-310) antibody
RUNX1 Reactivity: Human WB, ELISA, IF, IHC (p), PLA Host: Mouse Monoclonal 3A1 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (pSer304) antibody
RUNX1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) antibody
RUNX1 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 227-255) antibody
RUNX1 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) antibody
RUNX1 Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 5A1 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 237-337) antibody
RUNX1 Reactivity: Human ELISA, FACS Host: Mouse Monoclonal 6C12A5 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (Middle Region) antibody
RUNX1 Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:2000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date

6 months
Target

RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name

RUNX1

Background

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The RUNX1 protein represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis.

Molecular Weight

48737

Gene ID

861

NCBI Accession

NP_001001890, NP_001116079, NP_001745

UniProt

Q01196