UBR5 antibody

Catalog No. ABIN271946

The Rabbit Polyclonal anti-UBR5 antibody is suitable to detect UBR5 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for UBR5 antibody (ABIN271946)

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBR5 Antibody

Specificity: This antibody detects endogenous levels of EDD protein. (region surrounding Thr14)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Alternative Name: UBR5 / HYD

Background: EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth (2). EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.Synonyms: E3 ubiquitin-protein ligase, E3 ubiquitin-protein ligase UBR5, EDD, EDD1, HECT domain-containing 1, Hyperplastic discs protein homolog, KIAA0896

Molecular Weight: approx. 309 kDa

Gene ID: 51366

NCBI Accession: NP_056986

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway