XPA antibody

Catalog No. ABIN271978

This Rabbit Polyclonal antibody specifically detects XPA in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for XPA antibody (ABIN271978)

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XPA Antibody

Specificity: This antibody detects endogenous levels of XPA protein. (region surrounding His244)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000. Western blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for XPA

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Alternative Name: XPA / XPAC

Background: Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer due to deficiencies in the DNA repair enzymes. The most frequent mutations are found in the XP genes of group A through G and group V, which encode nucleotide excision repair proteins. The XPA gene encodes a 31 kDa zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair. The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (XPV) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.Synonyms: DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein

Molecular Weight: approx. 33, 40 kDa

Gene ID: 7507

NCBI Accession: NP_000371

UniProt: P23025

Pathways: DNA Damage Repair