Histone H2A antibody

Catalog No. ABIN271985

The Rabbit Polyclonal anti-Histone H2A antibody is suitable to detect Histone H2A in samples from Human, Mouse and Rat. It has been validated for IHC (p).

Quick Overview for Histone H2A antibody (ABIN271985)

Target: Histone H2A

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H2A Antibody

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/1000approx. 1/5000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Histone H2A

Target: Histone H2A

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Global mass spectrometric analysis of histone H2A variants/subtypes and their modifications have only recently been carried out. Nine histone H2A subtypes have been identified, including H2A2A and H2A2B. The two main H2A variants, H2AO and H2AC, as well as H2AL, were either acetylated at Lys 5 or phosphorylated at Ser 1. For the replacement histone H2AZ, acetylation at Lys 4 and Lys 7 was found.Synonyms: H2A Histone Family, HIST1H2A