Histone H2A antibody (ubLys119)

Catalog No. ABIN6971779

Product Details anti-Histone H2A Antibody

Target: Histone H2A

Binding Specificity: ubLys119

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Characteristics: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies. Histone H2AK119ub antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogen: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for Histone H2A

Target: Histone H2A

Abstract: Histone H2A Products

Synonyms: 5499 antibody, CG5499 antibody, Dmel\\CG5499 antibody, H2A antibody, H2A.F/Z antibody, H2A.X antibody, H2A.Z antibody, H2AV antibody, H2AV_DROM antibody, H2AX antibody, H2AZ antibody, H2Av antibody, H2AvD antibody, H2a.V antibody, H2av antibody, H2avD antibody, HIS antibody, HIS2AV antibody, HIS2AVD antibody, His antibody, His2 antibody, His2AV antibody, His2AvD antibody, HisH2Av antibody, Hist antibody, Hist2av antibody, gamma-H2Av antibody, gamma-HIS2AV antibody, gamma-His2Av antibody, gammaH2Av antibody, h2AvD antibody, his antibody, l(3)05146 antibody, l(3)810 antibody, l(3)97Dd antibody, l(3)L1602 antibody, DDBDRAFT_0205974 antibody, DDBDRAFT_0216271 antibody, DDB_0205974 antibody, DDB_0216271 antibody, RGD1564767 antibody, H2a-221 antibody, H2A-III antibody, HIST1H2AA antibody, Histone H2A variant antibody, putative histone H2A antibody, histone H2A antibody, histone cluster 1 H2A family member F antibody, histone H2A-like antibody, histone cluster 1, H2af antibody, histone2A1 antibody, histone cluster 1, H2A, III antibody, histone cluster 1, H2ac antibody, His2Av antibody, LMJF_17_0280 antibody, H2AX antibody, Hist1h2af antibody, LOC100425378 antibody, his2a1 antibody, HIST1H2A3 antibody, HIST1H2AC antibody, h2a-A antibody

Molecular Weight: 23 kDa

NCBI Accession: NP_003508