PEA15 antibody
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- Target See all PEA15 Antibodies
- PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PEA15 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of PEA-15 protein. (region surrounding Asp110)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product PEA15 Primary Antibody
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- Application Notes
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ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS, pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))
- Alternative Name
- PEA15 (PEA15 Products)
- Synonyms
- HMAT1 antibody, HUMMAT1H antibody, MAT1 antibody, MAT1H antibody, PEA-15 antibody, PED antibody, Pea15a antibody, PEA15 antibody, DKFZp459O1410 antibody, proliferation and apoptosis adaptor protein 15 antibody, phosphoprotein enriched in astrocytes 15 antibody, proliferation and apoptosis adaptor protein 15 L homeolog antibody, Astrocytic phosphoprotein PEA-15 antibody, PEA15 antibody, Pea15 antibody, pea15.L antibody, pea15 antibody
- Background
- PEA-15 (phosphoprotein enriched in astrocytes) exists in an non-phosphorylated form (N), and two phosphorylated forms, Pa and Pb. PEA-15 is an endogenous substrate for PKC, which mediates the transition from Pa to Pb. The level of PEA-15 phosphorylation changes upon depolymerization or stabilization of tubulins, indicating that PEA-15 co-localizes with microtubules. The first 80 amino acids of PEA-15 correspond to the death effector domain (DED), which is a domain found in proteins that regulate apoptotic signaling pathways. The DED domain is necessary for PEA-15 to block Ras suppression. Although PEA-15 is predominantly expressed in the central nervous system, low levels of PEA-15 are expressed in liver and kidney, and higher levels in muscle. PEA-15 is also referred to as PED, phosphoprotein enriched in diabetes, for its elevated expression in type 2 diabetic patients.Synonyms: Astrocytic phosphoprotein PEA-15, PED, Phosphoprotein enriched in diabetes
- Molecular Weight
- approx. 36 kDa
- Gene ID
- 8682
- NCBI Accession
- NP_003759
- UniProt
- Q15121
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