Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS) antibody

Antigen: Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS)

Synonyms: THC, IMD2, THC1, WASP

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN272092

Additional Information

Characteristics: Synonyms: Wiskott-Aldrich syndrome protein, WASp

Alternative name: WAS / IMD2

Swiss-Prot: P42768

Cross-Reactivity: Human, Mouse (Murine)

Description: The Wiskott-Aldrich syndrome (WAS) is a disorder that results from a monogenic defectthat has been mapped to the short arm of the X chromosome at Xp11. 23. WAS ischaracterized by thrombocytopenia, eczema, defects in cell-mediated and humoralimmunity and a propensity for lymphoproliferative disease. The gene that is mutated in thesyndrome encodes a 53 kDa proline-rich protein of unknown function designated WASprotein (WASP). A clue to WASP function came from the observation that T cells fromaffected males had an irregular cellular morphology and a disarrayed cytoskeletonsuggesting the involvement of WASP in cytoskeletal organization. Close examination of theWASP sequence revealed a putative Cdc42/Rac interacting domain, homologous withthose found in PAK65 and ACK. Subsequent investigation has shown WASP to be a truedownstream effector of Cdc42.

Specificity: This antibody detects endogenous levels of WASP protein. Species: Human, Mouse. Others not tested. Add. Information: Molecular Weight: ~60 kDa

Application Details

Application Notes: ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH~7.2 containing 15 mM Sdium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only