XIAP antibody

Catalog No. ABIN272094

The Rabbit Polyclonal anti-XIAP antibody (ABIN272094) specifically detects XIAP in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for XIAP antibody (ABIN272094)

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XIAP Antibody

Specificity: This antibody detects endogenous levels of XIAP protein. (region surrounding Gly81)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: IAP3 / BIRC4

Background: The human X chromosome linked Inhibitor of Apoptosis Protein (XIAP) is a major member of the inhibitor of apoptosis (IAP) family. XIAP functions by direct binding and inhibition of several caspases, including Caspase 3, Caspase 7 and Caspase 9. During apoptosis XIAP is specifically cleaved by Caspase 3 and partially loses its function. In addition to blocking the apoptosis signaling pathway, XIAP can also bind several other proteins in the bone morphogenetic protein (BMP) signaling pathway and act as a coupler between the membrane BMP receptor and the TAB1 TAK1 complex by binding to these proteins.Synonyms: API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, HILP, Inhibitor of apoptosis protein 3, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP

Molecular Weight: approx. 57 kDa

Gene ID: 331

NCBI Accession: NP_001158

UniProt: P98170

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis