HOXB9 antibody

Catalog No. ABIN272173

This Rabbit Polyclonal antibody specifically detects HOXB9 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for HOXB9 antibody (ABIN272173)

Target: HOXB9 (Homeobox B9 (HOXB9))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXB9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HOXB9 Antibody

Specificity: This antibody detects endogenous levels of Hox-B9 protein. (region surrounding Arg237)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for HOXB9

Target: HOXB9 (Homeobox B9 (HOXB9))

Alternative Name: HOXB9 / HOX2E

Background: The Hox proteins play a role in development and cellular differentiation by regulating downstream target genes. Specifically, the Hox proteins direct DNA-protein and protein-protein interactions that assist in determining the morphologic features associated with the anterior-posterior body axis. Themammalian Hox gene complex consists of 39 genes that are located on four linkage groups, which are dispersed over four chromosomes. Hox genes that occupy the same relative position along the 5' to 3' coordinate (trans-paralogous genes) are more similar in sequence and expression pattern than adjacent Hox genes on the same chromosome. In mice, the HoxB cluster contains HoxB1 to HoxB9 and HoxB13, which are transcribed in the same direction. HoxB9 associates with the transcriptional cofactors BTG1 and BTG2, which enhance HoxB9 ranscription. Alterations in HoxB9 expression, as with other Hox family member, has been implicated in leukemia.Synonyms: Homeobox protein Hox-B9, Hox-2.5, Hox-2E

Molecular Weight: approx. 28 kDa

Gene ID: 3219

NCBI Accession: NP_076922

UniProt: P17482