Septin 1 antibody

Catalog No. ABIN272245

This Rabbit Polyclonal antibody specifically detects Septin 1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Septin 1 antibody (ABIN272245)

Target: Septin 1 (SEPT1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Septin 1 Antibody

Specificity: This antibody detects endogenous levels of Septin 1 protein. (region surrounding Asp205)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Septin 1

Target: Septin 1 (SEPT1)

Alternative Name: Septin-1 (SEPT1)

Background: The septins are a family of GTPase enzymes, some of which are required for cytokinesis and others of which are associated with exocytosis. Members of the septin family can form heteropolymer complexes and also play a role in the organization of new growth in organisms. The transcriptional regulation of all septins is complex, resulting in alternatively spliced variants. At least three septins (Septin 1, 2 and 4) are associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Septin 3 (G-Septin), a GTP-binding protein, is highly expressed in brain and is regulated by protein kinase G in neurons. The human SEPT4 (H5/PNUTL2/CDCrREL-2) gene encodes ARTS (for apoptosis-related protein in the TGFβ signaling pathway), which is expressed in many cells and acts to enhance cell death induced by TGFβ or, to a lesser extent, by other apoptotic agents. ARTS is localized to mitochondria and translocates to the nucleus when apoptosis occurs.Synonyms: DIFF6, LARP, NY-BR-24, PNUTL3, Peanut-like protein 3, Serologically defined breast cancer antigen NY-BR-24

Molecular Weight: approx. 40 kDa

Gene ID: 1731

NCBI Accession: NP_443070

UniProt: Q8WYJ6