Serotonin Receptor 1A antibody

Catalog No. ABIN272268

The Rabbit Polyclonal anti-Serotonin Receptor 1A antibody (ABIN272268) specifically detects Serotonin Receptor 1A in WB, IF and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Serotonin Receptor 1A antibody (ABIN272268)

Target: Serotonin Receptor 1A (HTR1A)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 1A Antibody

Specificity: This antibody detects endogenous levels of SR-1A protein. (region surrounding Phe321)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 90 % pure SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Serotonin Receptor 1A

Target: Serotonin Receptor 1A (HTR1A)

Background: The members of the G protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. β-adrenergic bound to adrenaline activates adenylyl cyclase, while α2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the α2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories,designated SR-1-7 (5HT1-7). Subtypes within the SR-1 group include SR-1A,-1B, -1D, -1E and -1F.Synonyms: 5-HT-1A, 5-HT1A, 5-hydroxytryptamine receptor 1A, ADRB2RL1, ADRBRL1

Molecular Weight: approx. 46 kDa

Gene ID: 3350

NCBI Accession: NP_000515

UniProt: P08908

Pathways: JAK-STAT Signaling, Synaptic Membrane, Feeding Behaviour