CDC20 antibody

Catalog No. ABIN272282

The Rabbit Polyclonal anti-CDC20 antibody is suitable to detect CDC20 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for CDC20 antibody (ABIN272282)

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC20 Antibody

Specificity: This antibody detects endogenous levels of Cdc20 protein. (region surrounding Gln105)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CDC20

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Alternative Name: CDC20

Background: Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28. Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins,Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred.Synonyms: Cell division cycle protein 20 homolog, p55CDC

Molecular Weight: approx. 55 kDa

Gene ID: 991

NCBI Accession: NP_001246

UniProt: Q12834