ADD3 antibody

Catalog No. ABIN272287

This Rabbit Polyclonal antibody specifically detects ADD3 in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ADD3 antibody (ABIN272287)

Target: ADD3 (Adducin 3 (Gamma) (ADD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADD3 Antibody

Specificity: This antibody detects endogenous levels of Adducin γ protein. (region surrounding Pro452)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for ADD3

Target: ADD3 (Adducin 3 (Gamma) (ADD3))

Alternative Name: gamma-Adducin (ADD3)

Background: Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain andhematopoietic tissues, such as bone marrow in humans, and spleen in mice.Synonyms: ADDL, Adducin 3, Adducin-like protein 70

Molecular Weight: approx. 82 kDa

Gene ID: 120

NCBI Accession: NP_001112

UniProt: Q9UEY8