Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (pSer245) antibody

Antigen: Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4)

Synonyms: atf-4, MGC68539, zgc:85951, wu:fj01c08, wu:fk30b04, creb2, txreb, creb-2, MGC69565, taxreb67, ATF4, ATF4C, MGC126973, MGC179261, sb:eu681, wu:fb08f07, zgc:171702, CREB2

Epitope: pSer245

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN273315

Additional Information

Alternative name: ATF4 (Phospho-Ser245)

Swiss-Prot: P18848

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of serine 245 (N-R-S P -L-P).

Isotype: IgG  (Matching secondary antibodies)

Description: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity: ATF4 (phospho-Ser245) antibody detects endogenous levels of ATF4 only when phosphorylated at serine 245.

Application Details

Application Notes: IHC: 1:50~1:100

Concentration: 100µg/100µl

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: Store at -20° C /1 year

Restrictions: For Research Use only

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ATF4 (phospho-Ser245) antibody (E011053).