AMPK1 (Ab-174) antibody

Antigen: AMPK1 (Ab-174)

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN274033

Additional Information

Swiss-Prot: Q13131

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 ( L -R-T P -S-C).

Isotype: IgG  (Matching secondary antibodies)

Description: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity: AMPK1 (Ab-174) antibody detects endogenous levels of total AMPK1 protein.

Application Details

Application Notes: IHC: 1:50-1:100 WB: 1:500-1:1000 IF:1:100~1:200

Concentration: 100µg/100µl

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: Store at -20° C /1 year

Restrictions: For Research Use only

Images

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPK1 (Ab-174) antibody (E021191).

Western blot analysis of extracts from HT-29 cells, using AMPK1 (Ab-174) antibody (E021191, Lane 1 and 2).