HA-Tag antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Immunofluorescence (IF) (63), Flow Cytometry (FACS) (55), Western Blotting (WB) (44), ELISA (28), Immunoprecipitation (IP) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Dot Blot (Dot) (4), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (3), Chromatin Immunoprecipitation (ChiP) (1), Immunohistochemistry (IHC) (1), Immunostaining (ISt) (1)
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| Catalog no. |
ABIN274240 |
| Quantity |
50ug (100µg/100µl) (Variants) |
| Price |
226.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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The antiserum was produced by immunizing rabbit with synthesized peptide containing the influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Specificity
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HA-Tag polyclonal antibody detects HA-tagged proteins overexpressed in cells. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
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Application Notes
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WB: 1:500~1:1000
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Concentration
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100µg/100µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
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Buffer
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Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20° C /1 year
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Research Area
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Tags/Labels
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Restrictions
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For Research Use only
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Alternatives for antigen "HA-Tag", type "Antibodies"
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Hosts
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Rabbit (45), Mouse (43), Goat (6)
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Reactivities
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Fusion Protein (Tag) (42), Tag (14), All Species (12), Mouse (Murine) (4), Human (3), Rat (Rattus) (3)
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Applications
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Immunofluorescence (IF) (63), Flow Cytometry (FACS) (55), Western Blotting (WB) (44), ELISA (28), Immunoprecipitation (IP) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Dot Blot (Dot) (4), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (3), Chromatin Immunoprecipitation (ChiP) (1), Immunohistochemistry (IHC) (1), Immunostaining (ISt) (1)
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Conjugates
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Biotin (6), HRP (6), FITC (4), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
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Alternatives 
