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Flag-Tag antibody

Antigen

Flag-Tag

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Various Species

Application
Alternatives Western Blotting (WB)
Catalog no. ABIN274244
Quantity 50ug  (100µg/100µl)  (Variants)
Price 226.67 $   Plus shipping costs $35.00
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Additional Information

Immunogen The antiserum was produced by immunizing rabbit with synthesized peptide containing the Flag epitope (D-Y-K-D-D-D-D-K).
Isotype IgG  (Matching secondary antibodies)
Description Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Specificity Flag-Tag polyclonal antibody detects Flag-tagged proteins overexpressed in cells. The antibody recognizes the Flag-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.

Application Details

Application Notes WB: 1:500~1:1000
Concentration 100µg/100µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Buffer Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20° C /1 year
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Flag-Tag", type "Antibodies"
Hosts Mouse (2), Rabbit (1)
Reactivities Human (2), Mouse (Murine) (2), Rat (Rattus) (2), All Species (1)
Applications Western Blotting (WB) (3), Immunoprecipitation (IP) (2)