Recombinant DYKDDDDK Tag antibody (Cleavage Site)

Catalog No. ABIN7453373

The Rabbit Monoclonal anti-DYKDDDDK Tag antibody is suitable to detect DYKDDDDK Tag. It has been validated for WB, ELISA, IP and ICC.

Quick Overview for Recombinant DYKDDDDK Tag antibody (Cleavage Site) (ABIN7453373)

Target: DYKDDDDK Tag

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLRE00G

Product Details anti-DYKDDDDK Tag Antibody

Binding Specificity: Cleavage Site

Purpose: Rabbit anti-ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody [BLRE00G]

Purification: Purified

Immunogen: Enterokinase Cleavage Site (DYKDDDDK)

Isotype: IgG

Application Details

Application Notes:

IP: 20 μL/mg lysate

ICC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

ELISA: 1:1,000 - 1:75,000, for coating plates 1:100 - 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: ECS (DYKDDDDK)

Target Type: Tag

Background: The entereokinase cleavage site (ECS) tag is an epitope tag with the sequence DYKDDDDK. Its hydrophobic nature helps to maintain the structural integrity of the protein to which it is appended. Further, its nature as a kinase cleavage site allows for its removal from the recombinant protein if needed.