Biotin antibody

Catalog No. ABIN285011

The Rabbit Polyclonal anti-Biotin antibody is suitable to detect Biotin. It has been validated for WB and ELISA.

Quick Overview for Biotin antibody (ABIN285011)

Target: Biotin

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Biotin Antibody

Purification: Biotin antibody was purified by ion exchange chromatography followed by dialysis.

Immunogen: Biotin antibody was raised in rabbit using biotin conjugated to KLH as the immunogen.

Application Details

Application Notes: ELISA: 1:100,000, WB: 1:10,000-1:25,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Delipidated and defibrInated serum. Buffer contains 0.1 M Na3PO4, 0.11 M NaCl, pH 7.5 and 0.02 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: -20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.