This antibody reacts with a 30/32 kDa molecular weight lymphocyte surfaceantigen belonging to the CD8 cluster present on the human cytoxic/suppressor T cell subset.
Purification
Ion exchange chromatography
Immunogen
Carbonic anhydrase I antibody was raised in goat using carbonic anhydrase I from human Erythrocytes as the immunogen.
ELISA: 1:2,000-1:20,000, WB: 1:500-1:2,000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
Lot specific
Buffer
Lyophilized from 0.01 M Na1O4, pH 7.4, with 0.11 NaCl, and 0.01 % NaN3.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Target
CA1
(Carbonic Anhydrase I (CA1))
Alternative Name
Carbonic Anhydrase I
Background
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice-versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes.