Phosphotyrosine antibody

Catalog No. ABIN285915

The Rabbit Polyclonal anti-Phosphotyrosine antibody (ABIN285915) specifically detects Phosphotyrosine in WB, IP, IHC and ELISA.

Quick Overview for Phosphotyrosine antibody (ABIN285915)

Target: Phosphotyrosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA

Product Details anti-Phosphotyrosine Antibody

Cross-Reactivity (Details): Recognize proteins and peptides phosphorylated on tyrosine residues. Does not cross - react with phosphoserine and threonine.

Purification: Affinity purified

Immunogen: Phosphotyrosine antibody was raised in rabbit using Iodoacetylphosphotyrosine-KLH conjugate as the immunogen.

Application Details

Application Notes: ELISA: 1:1,000, IHC: 1:50, IP: 10 µg/500 µg protein sample, WB: 1:250
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Tris-acetate buffer, pH 7-7.5, with 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to phosphotyrosine which is considered as a key step in the regulation of enzymatic activities such as signal transduction.