Angiopoietin 2 antibody

Catalog No. ABIN289374

The Rabbit Polyclonal anti-Angiopoietin 2 antibody (ABIN289374) specifically detects Angiopoietin 2 in WB, IHC, ELISA and IF. The antibody is reactive with Mouse samples.

Quick Overview for Angiopoietin 2 antibody (ABIN289374)

Target: Angiopoietin 2 (ANGPT2)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Angiopoietin 2 Antibody

Purification: Affinity chromatography purified

Immunogen: Angiopoietin 2 antibody was raised in rabbit using 20-aa peptide from mouse Ang-2 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000- 1:100,000 (50-100 ng of control peptide/well), IHC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

By Western blot an antibody made to Ang-1 epitope has detected a approx. 70 kDa glycosylated, secreted protein in the conditioned media from cells expressing recombinant Ang-1.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.5, with 0.1 % BSA, and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: Angiopoietin 2

Background: Angiopoietin-2 is a homolog of Angiopoietin-1 (mouse and human Ang-2, 496 AA, ~85% identity) that has recently been identified. It may act an antagonist for Ang-1 and the Tie-2 receptor. Ang-1 and Ang-2 have ~60% sequence homology.It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

Pathways: RTK Signaling