Fibronectin 1 (FN1) antibody

Antigen: Fibronectin 1 (FN1)

Synonyms: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, DKFZp686H0342, DKFZp686I1370, DKFZp686F10164, DKFZp686O13149, Fn, Fn-1, MGC117493, E330027I09, fn-1, FIBNEC, FN1, cig, msf, finc, lets, MGC97508, fibronectin, fn2, fb80d10, wu:fb80d10

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN289427

Additional Information

Alternative name: Fibronectin

Immunogen: Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Description: Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.

Specificity: This antibody reacts with Human Fibronectin. Ths antibody has negligible cross reactivity with Type I,II,III,IV,V,VI Collagens or Laminin1.0 mg/ml

Synonyms: FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, DKFZp686H0342, DKFZp686I1370, DKFZp686F10164, DKFZp686O13149, Fn, Fn-1, MGC117493, E330027I09, fn-1, FIBNEC, FN1, cig, msf, finc, lets, MGC97508, fibronectin, fn2, fb80d10, wu:fb80d10

Application Details

Concentration: 1.0 mg/ml

Purity: Affinity Purified

Purification: Fibronectin antibody was purified by affinity chromatography.

Buffer: Sterile filtered liquid in 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.

Storage: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage. Avoid repeated freeze/thaw cycles.

Research Area: Lineage Markers, Extracellular Matrix