CYP1B1 antibody (AA 400-450)

Catalog No. ABIN290263

This Rabbit Polyclonal antibody specifically detects CYP1B1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Monkey and Chicken.

Quick Overview for CYP1B1 antibody (AA 400-450) (ABIN290263)

Target: CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYP1B1 Antibody

Binding Specificity: AA 400-450

Specificity: A portion of amino acids 400-450 of human CYP1B1.

Purification: Protein G purified

Immunogen: A portion of amino acids 400-450of human CYP1B1.

Type of Immunogen: Protein fragment

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CYP1B1

Target: CYP1B1 (Cytochrome P450, Family 1, Subfamily B, Polypeptide 1 (CYP1B1))

Alternative Name: CYP1B1

Background: Name/Gene ID: CYP1B1
Family: Cytochrome P450

Synonyms: CYP1B1, Aryl hydrocarbon hydroxylase, CP1B, Cytochrome P450 1B1, CYPIB1, GLC3A, p4501B1

Gene ID: 1545

UniProt: Q16678

Pathways: Steroid Hormone Biosynthesis