IDO1 antibody (AA 129-141)

Catalog No. ABIN292259

The Goat Polyclonal anti-IDO1 antibody is suitable to detect IDO1 in samples from Human, Mouse and Monkey. It has been validated for ELISA, IHC and IHC (p).

Quick Overview for IDO1 antibody (AA 129-141) (ABIN292259)

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivity: Human, Mouse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IDO1 Antibody

Binding Specificity: AA 129-141

Specificity: Human IDO1 / IDO.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon, Mouse, Rat, Sheep, Dog, Bovine, Panda (92%) Bat, Elephant, Horse, Rabbit, Pig, Opossum, Platypus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CVLANWKKKDPNK from an internal region of human IDO1 / IDO (NP_002155.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon, Mouse, Rat, Sheep, Dog, Bovine, Panda (92%), Bat, Elephant, Horse, Rabbit, Pig, Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO1 / IDO

Background: Name/Gene ID: IDO1

Synonyms: IDO1, IDO, Indole 2,3-dioxygenase, Indolamine 2,3 dioxygenase, IDO-1, INDO, Indoleamine 2,3-dioxygenase, Indoleamine 2,3-dioxygenase 1

Gene ID: 3620

NCBI Accession: NP_002155

UniProt: P14902

Pathways: Activated T Cell Proliferation