SMAD2 antibody (AA 234-249)

Catalog No. ABIN293877

The Rabbit Polyclonal anti-SMAD2 antibody (ABIN293877) specifically detects SMAD2 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Chicken, Monkey, Dog, Horse, Bat, Hamster and Xenopus laevis samples.

Quick Overview for SMAD2 antibody (AA 234-249) (ABIN293877)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Chicken, Monkey, Dog, Horse, Bat, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMAD2 Antibody

Binding Specificity: AA 234-249

Specificity: The SMAD2 amino acid sequence used as immunogen is 100 % homologous in human (isoform 1:467 amino acids, isoform 2:437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%) Panda, Stickleback, Pufferfish, Zebrafish (94%).

Purification: Protein G purified

Immunogen: Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 were used as immunogen. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Rabbit, Horse, Opossum, Chicken, Platypus, Lizard, Xenopus (100%), Panda, Stickleback, Pufferfish, Zebrafish (94%).

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background: Name/Gene ID: SMAD2

Synonyms: SMAD2, HMAD-2, HSMAD2, JV18-1, Mad protein homolog smad2, MADR2, MADH2, SMAD 2, MAD homolog 2, Mad-related protein 2, JV18, Mother against DPP homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD family member 2

Gene ID: 4087

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus