RHPN2 antibody (AA 645-655)

Catalog No. ABIN294091

This Goat Polyclonal antibody specifically detects RHPN2 in ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Cow, Rabbit, Bat and Monkey.

Quick Overview for RHPN2 antibody (AA 645-655) (ABIN294091)

Target: RHPN2 (Rhophilin, rho GTPase Binding Protein 2 (RHPN2))

Reactivity: Human, Cow, Rabbit, Bat, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This RHPN2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RHPN2 Antibody

Binding Specificity: AA 645-655

Specificity: Human RHPN2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Bat, Bovine, Elephant, Panda, Rabbit, Opossum, Platypus (100%) Mouse, Rat, Hamster, Horse (91%) Dog (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TNKNRQKSAST from an internal region of human RHPN2 (NP_149094.3). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Bat, Bovine, Elephant, Panda, Rabbit, Opossum, Platypus (100%), Mouse, Rat, Hamster, Horse (91%), Dog (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RHPN2

Target: RHPN2 (Rhophilin, rho GTPase Binding Protein 2 (RHPN2))

Alternative Name: RHPN2

Background: Name/Gene ID: RHPN2

Synonyms: RHPN2, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, Rhophilin-2, p76RBE, RHOBP, Rhophilin 2

Gene ID: 85415

NCBI Accession: NP_149094

UniProt: Q8IUC4