HDAC4 antibody (AA 194-209)

Catalog No. ABIN294416

The Rabbit Polyclonal anti-HDAC4 antibody is suitable to detect HDAC4 in samples from Human and Mouse. It has been validated for WB, IHC, IP, IHC (p) and ChIP.

Quick Overview for HDAC4 antibody (AA 194-209) (ABIN294416)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Product Details anti-HDAC4 Antibody

Binding Specificity: AA 194-209

Specificity: A 140 kD band is observed.

Purification: Protein G purified

Immunogen: Synthetic peptide from human HDAC4.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ChrIP, IHC, IHC-P (5 μg/mL), IP, WB (5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4

Background: Name/Gene ID: HDAC4
Family: Histone Deacetylase

Synonyms: HDAC4, BDMR, AHO3, Histone deacetylase A, HD4, HA6116, HDAC-A, HDACA, Histone deacetylase 4, HDAC-4, KIAA0288

Gene ID: 9759

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process