Blood Group Lewis A antibody

Catalog No. ABIN294426

Product Details

Target: Blood Group Lewis A

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Specificity: Recognizes Lewis a blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal tumors

Purification: Protein A purified

Immunogen: Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification).

Type of Immunogen: Purified protein

Clone: 7LE

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, WB

Usage: Immunohistochemistry (paraffin sections): Recommended dilution - 5 μg/mL. Positive control - colorectal adenocarcinoma. Application note - heat mediated antigen retrieval (sodium citrate). mAb incubation 1 hour / RT. detection DAB.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: Blood Group Lewis A

Alternative Name: Lewis A Blood Group Antigen

Synonyms: CD174 antibody, FT3B antibody, FucT-III antibody, LE antibody, Les antibody, fucosyltransferase 3 (Lewis blood group) antibody, FUT3 antibody