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Blood Group Lewis A antibody

Reactivity: Human IHC (p), WB, ELISA Host: Mouse Monoclonal 7LE unconjugated
Catalog No. ABIN93924
  • Target
    Blood Group Lewis A
    Reactivity
    • 29
    • 10
    Human
    Host
    • 16
    • 13
    Mouse
    Clonality
    • 16
    • 13
    Monoclonal
    Conjugate
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 19
    • 13
    • 13
    • 11
    • 11
    • 9
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
    Specificity
    The mouse monoclonal antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed on colonic epithelial cells. Lewisa may be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
    Cross-Reactivity (Details)
    Other not tested, Human
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
    Clone
    7LE
    Isotype
    IgG1
  • Application Notes
    Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive control: colorectal adenocarcinoma, heat mediated antigen retrieval (sodium citrate), mAb incubation 1 hour / RT, detection DAB.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Creuzot-Garcher, Guerzider, Assem, Bron, Delannoy, Bara: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).

    "First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems." in: Revue française de transfusion et immuno-hématologie, Vol. 31, Issue 2, pp. 115-486, (1989) (PubMed).

  • Target
    Blood Group Lewis A
    Synonyms
    CD174 antibody, FT3B antibody, FucT-III antibody, LE antibody, Les antibody, fucosyltransferase 3 (Lewis blood group) antibody, FUT3 antibody
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