HIPK3 antibody (AA 850-900)

Catalog No. ABIN295864

This Rabbit Polyclonal antibody specifically detects HIPK3 in IHC, WB and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for HIPK3 antibody (AA 850-900) (ABIN295864)

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIPK3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HIPK3 Antibody

Binding Specificity: AA 850-900

Specificity: A portion of amino acids 850-900 of human HIPK3.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 850-900of human HIPK3.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HIPK3

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Alternative Name: HIPK3 / FIST

Background: Name/Gene ID: HIPK3
Subfamily: MNB/DYRK
Family: Protein Kinase

Synonyms: HIPK3, ANPK, FIST, FIST3, Homolog of protein kinase YAK1, DYRK6, PKY, YAK1

Gene ID: 10114

UniProt: Q9H422