ATG5 antibody (AA 1-50)

Catalog No. ABIN296750

The Rabbit Polyclonal anti-ATG5 antibody is suitable to detect ATG5 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Pig and Tetraodontidae (Puffer Fish). It has been validated for IHC and IHC (p).

Quick Overview for ATG5 antibody (AA 1-50) (ABIN296750)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Pig, Tetraodontidae (Puffer Fish)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG5 Antibody

Binding Specificity: AA 1-50

Specificity: A portion of amino acid 1-50 of human APG5L

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human ATG5.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5 / ATG5

Background: Name/Gene ID: ATG5

Synonyms: ATG5, APG5, APG5-LIKE, APG5L, Apoptosis specific protein, Apoptosis-specific protein, Autophagy related 5, HAPG5, ASP, Autophagy protein 5

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy