HTRA3 antibody (AA 250-300)

Catalog No. ABIN296848

This Rabbit Polyclonal antibody specifically detects HTRA3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat and Monkey.

Quick Overview for HTRA3 antibody (AA 250-300) (ABIN296848)

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTRA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HTRA3 Antibody

Binding Specificity: AA 250-300

Specificity: The amino acid sequence is 100 % homologous in HtrA3, 80 % homologous in HtrA1 and 71 % homologous in HtrA2 and HtrA4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%) Gorilla, Bovine, Pig, Pufferfish (93%) Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%) Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).

Purification: Protein G purified

Immunogen: Amino acids 273-287 (IVSTAQREGRELGLR)of human HtrA3 were used as immunogen. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%), Gorilla, Bovine, Pig, Pufferfish (93%), Mouse, Rat, Panda, Dog, Horse, Turkey, Chicken, Xenopus, Stickleback (87%), Hamster, Elephant, Opossum, Guinea pig, Platypus, Lizard, Salmon, Zebrafish (80%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HTRA3

Target: HTRA3 (HtrA Serine Peptidase 3 (HTRA3))

Alternative Name: HTRA3

Background: Name/Gene ID: HTRA3
Subfamily: Serine S1C
Family: Protease

Synonyms: HTRA3, HtrA serine peptidase 3, Serine protease HTRA3, Tasp, Probable serine protease HTRA3, PRSP

Gene ID: 94031

UniProt: P83110