IRAK4 antibody (AA 38-54)

Catalog No. ABIN297197

The Rabbit Polyclonal anti-IRAK4 antibody is suitable to detect IRAK4 in samples from Human and Mouse. It has been validated for WB, IHC and IHC (p).

Quick Overview for IRAK4 antibody (AA 38-54) (ABIN297197)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRAK4 Antibody

Binding Specificity: AA 38-54

Specificity: A mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4.

Purification: Protein G purified

Immunogen: Synthetic peptide from mouse IRAK4.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4 / IRAK-4

Background: Name/Gene ID: IRAK4
Subfamily: IRAK(Pelle)
Family: Protein Kinase

Synonyms: IRAK4, IPD1, IRAK-4, NY-REN-64, REN64

Gene ID: 51135

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades