RICTOR antibody (AA 1722-1732)

Catalog No. ABIN297558

The Goat Polyclonal anti-RICTOR antibody (ABIN297558) specifically detects RICTOR in ELISA, IHC and IHC (p). The antibody is reactive with Human, Monkey, Dog and Horse samples.

Quick Overview for RICTOR antibody (AA 1722-1732) (ABIN297558)

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reactivity: Human, Monkey, Dog, Horse

Host: Goat

Clonality: Polyclonal

Conjugate: This RICTOR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RICTOR Antibody

Binding Specificity: AA 1722-1732

Specificity: Human RICTOR.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Horse (100%) Elephant, Bovine, Pig, Opossum (91%) Mouse, Rat, Panda, Bat, Blood fluke (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KQPIVDTSAES from the C-terminus of human RICTOR (NP_689969.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Horse (100%), Elephant, Bovine, Pig, Opossum (91%), Mouse, Rat, Panda, Bat, Blood fluke (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:4000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RICTOR

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Alternative Name: RICTOR

Background: Name/Gene ID: RICTOR

Synonyms: RICTOR, AVO3 homolog, HAVO3, MAVO3, PIA, KIAA1999, TORC2-specific protein AVO3, AVO3, Pianissimo

Gene ID: 253260

NCBI Accession: NP_689969

UniProt: Q6R327

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events