SEMA3E antibody (AA 762-775)

Catalog No. ABIN297629

The Goat Polyclonal anti-SEMA3E antibody is suitable to detect SEMA3E in samples from Human. It has been validated for IHC, ELISA and IHC (p).

Quick Overview for SEMA3E antibody (AA 762-775) (ABIN297629)

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SEMA3E antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SEMA3E Antibody

Binding Specificity: AA 762-775

Specificity: Human SEMA3E.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Horse (93%) Hamster, Elephant, Panda, Bat, Bovine, Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KPEHYRLPRHTLDS from the C-terminus of human SEMA3E (NP_036563.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Horse (93%), Hamster, Elephant, Panda, Bat, Bovine, Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SEMA3E

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Alternative Name: Semaphorin 3E / SEMA3E

Background: Name/Gene ID: SEMA3E

Synonyms: SEMA3E, M-SEMAH, SEMAH, Semaphorin 3E, M-SemaK, Semaphorin-3E, Coll-5, KIAA0331

Gene ID: 9723

NCBI Accession: NP_036563

UniProt: O15041