RNF2 antibody (AA 189-201)

Catalog No. ABIN297805

This Goat Polyclonal antibody specifically detects RNF2 in WB, ELISA, IF, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Chicken and Frog.

Quick Overview for RNF2 antibody (AA 189-201) (ABIN297805)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Frog

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RNF2 Antibody

Binding Specificity: AA 189-201

Specificity: Aa 189-201 of human RING1B protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human RNF2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: RNF2 / RING2 / RING1B

Background: Name/Gene ID: RNF2

Synonyms: RNF2, DING, HIP2-interacting protein 3, Protein DinG, Ring finger protein 2, RING finger protein BAP-1, BAP-1, HIPI3, RING finger protein 1B, RING1B, RING2

Gene ID: 6045

UniProt: Q99496