SRD5A1 antibody (AA 2-13)

Catalog No. ABIN297922

This Goat Polyclonal antibody specifically detects SRD5A1 in IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SRD5A1 antibody (AA 2-13) (ABIN297922)

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRD5A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SRD5A1 Antibody

Binding Specificity: AA 2-13

Specificity: Human SRD5A1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla, Marmoset (92%) Ajellomyces (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide ATATGVAEERLLC from the N-terminus of human SRD5A1 (NP_001038.1). Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla, Marmoset (92%), Ajellomyces (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SRD5A1

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Alternative Name: 5-Alpha Reductase / SRD5A1

Background: Name/Gene ID: SRD5A1

Synonyms: SRD5A1, 5-alpha reductase, SR type 1, Steroid 5-alpha-reductase 1, S5AR 1

Gene ID: 6715

NCBI Accession: NP_001038

UniProt: P18405

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process