Caspase 7 antibody

Catalog No. ABIN298976

The Mouse Monoclonal anti-Caspase 7 antibody is suitable to detect Caspase 7 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for Caspase 7 antibody (ABIN298976)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 25B881-1

Product Details anti-Caspase 7 Antibody

Specificity: In Jurkat, a 35 kD band is observed.

Purification: Protein G purified

Immunogen: Recombinant human Caspase-7.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.2 % gelatin and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: CASP7 / Caspase 7

Background: Name/Gene ID: CASP7
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP7, Apoptotic protease Mch-3, Caspase 7, Caspase-7, ICE-LAP3, LICE2, MCH3, ICE-like apoptotic protease 3, CASP-7, CMH-1

Gene ID: 840

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity