Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (HRP)

Catalog No. ABIN301109

Quick Overview for Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (HRP) (ABIN301109)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Pig

Host: Rabbit

Specificity: Anti-Peroxidase, anti-Rabbit Serum, Swine IgG F(ab')2 and Swine Serum. No reaction was seen against Swine IgG F(c)

Purification: Immunoaffinity purified

Immunogen: Pig IgG F(ab')2f fragment.

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-anti body based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Swine IgG F(ab')2 in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.01 M sodium phosphate, 0.14 M sodium chloride,  pH 7.4, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Porcine IgG Fab'2, Swine IgG Fab'2