Goat anti-Rat IgG (Fc Region) Antibody (Biotin)

Catalog No. ABIN301468

Quick Overview for Goat anti-Rat IgG (Fc Region) Antibody (Biotin) (ABIN301468)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Rat

Host: Goat

Specificity: Anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab')2 or Bovine, Horse and Human Serum Proteins

Purification: Immunoaffinity purified

Immunogen: Rat IgG Fc fragment.

Application Details

Application Notes: Approved: ELISA (1:15000 - 1:90000), IHC, WB

Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Target Details

Target: IgG

Target Type: Antibody