CYP2E1 antibody (C-Term)

Catalog No. ABIN301632

The Rabbit Polyclonal anti-CYP2E1 antibody (ABIN301632) specifically detects CYP2E1 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CYP2E1 antibody (C-Term) (ABIN301632)

Target: CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP2E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYP2E1 Antibody

Binding Specificity: C-Term

Specificity: Synthetic peptide corresponding to C-terminal residues of human CYP2E1 (Cytochrome P450 2E1)

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human CYP2E1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1 μg/mL), IHC, IHC-P (20 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for CYP2E1

Target: CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))

Alternative Name: CYP2E1

Background: Name/Gene ID: CYP2E1
Family: Cytochrome P450

Synonyms: CYP2E1, 4-nitrophenol 2-hydroxylase, CPE1, Cytochrome p450 IIe1, Cytochrome P450-J, p450C2E, p450-J, CYP2E, CYPIIE1, Cytochrome P450 2E1

Gene ID: 1571

UniProt: P05181