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Antibody
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anti-Cytokeratin 18 KRT18 FITC
Cytokeratin 18 (KRT18) antibody (FITC)
| Antigen |
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| Synonyms |
K18, CK18, Krt1-18, CYK18 |
| Clonality |
Monoclonal (DC-10) |
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Host
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Reactivity
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Human (133), Rat (Rattus) (48), Mouse (Murine) (47), Dog (Canine) (39), Rabbit (33), Cow (Bovine) (21), Pig (Porcine) (21), Chicken (18), Horse (Equine) (15), Mammalian (11), Hamster (5), None (4), Sheep (Ovine) (3), Trout (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1), Zebrafish (Danio rerio) (1)
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Conjugate
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FITC (12), Biotin (11), HRP (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1)
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Application
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Western Blotting (WB) (79), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Immunofluorescence (IF) (59), Flow Cytometry (FACS) (56), Immunohistochemistry (IHC) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (23), ELISA (20), Immunoprecipitation (IP) (19), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (2)
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| Catalog no. |
ABIN302009 |
| Quantity |
0.1 mg (0.1 mg/ml) |
| Price |
344.50 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Alternative name
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Cytokeratin 18
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Immunogen
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Human breast carcinoma cell line PMC-42.
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Isotype
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IgG1
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Clone
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DC-10
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Description
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Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).
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Specificity
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The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Dog (Canine) .
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Application Notes
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The reagent is designed for Flow Cytometry analysis and Immunofluorescence. Suggested working dilution is 5-10 ug/ml. Indicated dilution is recommended starting pointfor use of this product. Working concentrations should be determined by the investigator.
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Concentration
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0.1 mg/ml
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with Fluorescein isothiocy
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Storage
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Research Area
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Signaling, Cytoskeleton, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
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Hosts
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Mouse (93), Rabbit (66), Guinea Pig (1), Sheep (1)
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Reactivities
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Human (133), Rat (Rattus) (48), Mouse (Murine) (47), Dog (Canine) (39), Rabbit (33), Cow (Bovine) (21), Pig (Porcine) (21), Chicken (18), Horse (Equine) (15), Mammalian (11), Hamster (5), None (4), Sheep (Ovine) (3), Trout (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (79), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Immunofluorescence (IF) (59), Flow Cytometry (FACS) (56), Immunohistochemistry (IHC) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (23), ELISA (20), Immunoprecipitation (IP) (19), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (2)
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Conjugates
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FITC (12), Biotin (11), HRP (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1)
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Epitopes
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C-Term (5), pSer33 (5), Center (1), N-Term (1)
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Alternatives 
