V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) antibody (FITC)

Antigen: V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyms: MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746

Clonality: Monoclonal (9E10)

Host: Mouse

Reactivity: Human, All Species

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302017

Additional Information

Alternative name: c-Myc

Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region ofhuman c-Myc.

Isotype: IgG1

Clone: 9E10

Specificity: The antibody 9E10 may be used to detect the c-Myc tag.The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-mycgene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is atranscription factor (nuclear localization). c-Myc is commonly activated in a variety of tumorcells and plays an important role in cellular proliferation, differentiation, apoptosis and cellcycle progression. The phosphorylation of c-Myc has been investigated and previousstudies have suggested a functional association between phosphorylation at Thr58/Ser62 byglycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase(JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc istightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is alsoessential for tumor cell development in vasculogenesis and angiogenesis that distributeblood throughout the cells.

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 200.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. Application note: Membrane permeabilization is required.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Cancer, Cell Cycle, Transcription Factors

Restrictions: For Research Use only

Publications

Publications: Hilpert, Hansen, Wessner et al.: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001 (PubMed).

Baggio, Burgstaller, Hale et al.: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002 (PubMed).

Fujiwara, Poikonen, Aleman et al.: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002 (PubMed).

Wang, Campoli, Ko et al.: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004 (PubMed).

Veracini, Simon, Richard et al.: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008 (PubMed).