Myc Tag antibody (AA 423-435)
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- Target See all Myc Tag products
- Myc Tag
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Binding Specificity
- AA 423-435
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Myc Tag antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Brand
- IHC-plus™
- Specificity
- The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Orangutan, Monkey, Marmoset, Tamarin (92%) Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
- Purification
- Protein G purified
- Immunogen
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Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Monkey, Marmoset, Tamarin (92%), Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
Type of Immunogen: Synthetic peptide - Clone
- 9E10
- Isotype
- IgG1
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- Application Notes
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Approved: Flo (1 - 5 μg/mL), IHC, IHC-P (10 μg/mL), IP (1 - 5 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 15 mM sodium azide, approx, pH 7.4.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- Myc Tag
- Abstract
- Myc Tag Products
- Target Type
- Tag
- UniProt
- P01106
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